Studies on Bacteriophage M13 DNA. 1. A Cleavage Map of the M13 Genome
نویسندگان
چکیده
منابع مشابه
Electron microscopic studies of bacteriophage M13 DNA replication.
Intracellular forms of M13 phage DNA isolated after infection of Escherichia coli with wild-type phage have been studied by electron microscopy and ultracentrifugation. The data indicate the involvement of rolling-circle intermediates in single-stranded DNA synthesis. In addition to single-stranded circular DNA, we observed covalently closed and nicked replicative-form (RF) DNAs, dimer RF DNAs,...
متن کاملConformations of the single-stranded DNA of bacteriophage M13.
At least two conformations of M13 single-stranded DNA have been demonstrated by measuring differences in sedimentation coefficient and by direct visualization in the electron microscope. Which form is obtained from infected cells and/or intact phage depends on the pH, ionic strength, and temperature. The slower-sedimenting form can be converted to the faster-sedimenting, single-stranded form by...
متن کاملPhysical mapping of the central terminator for transcription on the bacteriophage M13 genome.
With the aid of in vitro transcription and translation studies it has been demonstrated that termination of transcription on bacteriophage M13 replicative form DNA occurs at a unique site which is located immediately distal to the 3'-end of gene VIII, the gene which codes for the major capsid protein. The position of this site has been mapped accurately on the enzyme cleavage maps by transcript...
متن کاملCell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody
Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-G...
متن کاملOriented cell growth on self-assembled bacteriophage M13 thin films.
Fibrillar M13 bacteriophages were used as basic building blocks to generate thin films with aligned nanogrooves, which, upon chemical grafting with RGD peptides, guide cell alignment and orient the cell outgrowth along defined directions.
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1975
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1975.tb04098.x